论文部分内容阅读
目的：观察小鼠卡氏肺孢子虫性肺炎（ＰＣＰ）病原和病理学改变。方法：昆明小鼠皮下注射醋酸考的松诱发ＰＣＰ。肺印片Ｇｉｅｍｓａ染色检查卡氏肺孢子虫包囊；常规石蜡切片，ＨＥ梁色、ＰＡＳ染色及ＧＭＳ染色观察病理学改变。结果：自用药第５周肺印片即可检出卡氏肺孢子虫包囊，第８周包囊检出率达８１．８２％；ＨＥ染色切片早期呈以淋巴细胞、单核细胞渗出为主的间质性肺炎；晚期间质性肺炎加重，肺泡腔内出现泡沫样渗出物；ＰＡＳ染色切片泡沫样渗出物呈桃红色阳性反应；ＧＭＳ染色切片可见染成黑色的包囊。结论：本研究为建立ＰＣＰ动物模型提供了病原形态学和病理学依据；同时所显示的ＰＣＰ病理变化对临床病理工作者有较好的诊断参考价值
Objective: To observe the pathogenic and pathological changes of Pneumocystis carinii pneumonia (PCP) in mice. Methods: Kunming mice subcutaneous injection of testosterone acetate induced PCP. Pneumocystis carinii pneumocystis was examined by Giemsa staining. Pathological changes were observed by paraffin sections, HE beam color, PAS staining and GMS staining. Results: Cysts of Pneumocystis carinii were detected in the lungs of the fifth week of application, and the detection rate of cysts was 81.82% at the 8th week. The early stage of HE staining showed the exudation of lymphocytes and monocytes Mainly interstitial pneumonia; late interstitial pneumonia aggravated, alveolar effusion foam-like exudate; PAS staining of foam-like exudate pink positive reaction; GMS stained sections can be seen dyed black cysts. Conclusion: This study provides the basis for pathogenic morphology and pathology in establishing animal model of PCP. At the same time, the pathological changes of PCP showed a good diagnostic value for clinicopathologists