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目的:探究过氧化物酶6(peroxiredoxin 6,Prdx6)对小鼠深Ⅱ度烫伤创面愈合修复作用及其作用机制。方法:昆明种小鼠90只,雌雄各半,实验前24 h进行硫化钠脱毛处理,取放置于沸水中15 min边长为3 cm的正方体铁块,紧贴小鼠背部10 s,建立小鼠深Ⅱ度烫伤模型,随机分为模型组(PBS),实验组(Prdx6),阳性对照组(外用重组人碱性成纤维细胞生长因子,rh-b FGF),每组30只,qid,连续给药21 d。观察记录小鼠皮肤创面愈合时间,计算创面愈合率。于烫伤后5,10,14 d,HE染色观察创面病理学改变,对创面组织丙二醛(malondialdehyde,MDA)及超氧化物歧化酶(superoxide dismutase,SOD)含量进行测定,采用逆转录聚合酶链反应(RT-PCR)检测烫伤皮肤组织肿瘤坏死因子-α(TNF-α)及白细胞介素1-β(IL-1β)的mRNA表达水平。结果:与模型组相比,Prdx6组小鼠愈合时间短,结痂面积小,创面愈合率较高,皮肤水肿程度较轻。Prdx6可降低MDA含量,升高SOD含量,降低不同时间点的TNF-α与IL-1βmRNA表达量。结论:Prdx6对小鼠深Ⅱ度烫伤创面愈合具有很好的修复作用。其作用机制可能与降低皮肤损伤过程中脂质过氧化反应,保护SOD等抗氧化酶活性,降低TNF-α与IL-1β表达,减轻氧化应激及局部炎症反应对皮肤的损伤等有关。
Objective: To investigate the effect and mechanism of peroxiredoxin 6 (Prdx6) on wound healing in deep second degree scalded mice. METHODS: Ninety Kunming mice, male and female, were treated with sodium sulfide hair removal treatment 24 hours before the experiment. Square iron blocks with a side length of 3 cm placed in boiling water for 15 min were attached to the back of the mice for 10 s to establish a small Rats were randomly divided into three groups: model group (PBS), experimental group (Prdx6), positive control group (rhBFGF, rh-b FGF) Continuous administration of 21 d. Observe the wound healing time of the mouse skin to calculate the wound healing rate. At 5, 10 and 14 days after scald, the pathological changes of the wounds were observed by HE staining. The content of malondialdehyde (MDA) and superoxide dismutase (SOD) in the wound tissues were determined. Reverse transcription polymerase The mRNA expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in scalded skin tissues were detected by RT-PCR. Results: Compared with the model group, Prdx6 mice had shorter healing time, smaller scab area, higher wound healing rate and less skin edema. Prdx6 can reduce the content of MDA, increase the content of SOD and decrease the expression of TNF-α and IL-1βmRNA at different time points. Conclusion: Prdx6 has a good repair effect on deep partial thickness scald wound healing in mice. The mechanism may be related to the reduction of lipid peroxidation during skin lesion, protection of antioxidant enzymes such as SOD, decrease of TNF-α and IL-1β expression, alleviation of oxidative stress and local inflammatory reaction on skin damage.