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目的 :探讨大肠癌细胞 Fas配体 (Fas L ) m RNA和蛋白的表达及其功能。方法 :分别采用 RT- PCR和 Western印迹法以及氚释放细胞活性测定技术检测大肠癌细胞株中 Fas L m RNA和蛋白的表达及其功能。结果 :所检测的 6株大肠癌细胞(RPMI 4788、HCT- 116、DL D- 1、L o Vo、Wi Dr和 COL O 32 0 DM)全部表达 Fas L m RNA和蛋白 ;DL D- 1,L o Vo和 Wi Dr等部分大肠癌细胞具有 Fas依赖的细胞毒活性。其中 DL D- 1细胞活性具有量效关系 ,并且乙酸肉豆蔻佛波醇 (PMA )和离子霉素(ionom ycine )刺激能显著增强 DL D- 1的细胞活性。结论 :DL D- 1、L o Vo和 Wi Dr等 3株大肠癌细胞表达功能性 Fas配体 ,并可能以此反击机体免疫系统 ,逃避免疫监督。
Objective: To investigate the expression and function of Fas L m RNA and protein in colorectal cancer cells. METHODS: The expression and function of Fas L mRNA and protein in colorectal cancer cell lines were detected by RT-PCR and Western blotting, respectively. Results: All 6 colorectal cancer cells (RPMI 4788, HCT-116, DL D-1, L o Vo, Wi Dr and COL O 32 0 DM) expressed all expressed Fas L m RNA and protein; DL D-1 Some colon cancer cells such as L o Vo and Wi Dr have Fas-dependent cytotoxic activity. The activity of DL D-1 cells has a dose-effect relationship, and the stimulation of Pharmacopoeia acetate (PMA) and ionom ycine can significantly enhance the cell activity of DL D-1. Conclusions: Three colorectal cancer cells, DL D-1, Lo Vo, and Wi Dr, expressed functional Fas ligands, which may counterattack the immune system and evade immune surveillance.