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输血及静注毒品成瘾是丙型肝炎病毒(HCV)传播的主要原因。最近血清学调查资料表明,HCV可通过母亲垂直传播给婴儿。作者用聚合酶链反应(PCR)法调查了HCV的母婴传播,并对感染患儿的病程进行了观察。受试者为有胃肠外或性传播疾病,并在艾滋病中心住院的10例高危孕妇。在无菌条件下采血,脐带血用血管导管采集以免被母体血污染。血浆用Ortho抗-HCV酶免疫试验(EIA)检测,并用Chiroa重组免疫印迹试验(RIBA)重复检测。在孕妇临近分娩或分娩时检测ALT,婴儿于4～6月龄
Blood transfusion and intravenous drug addiction are the major causes of the spread of hepatitis C virus (HCV). Recent serological survey data indicate that HCV can be transmitted vertically to the baby by her mother. The authors investigated the mother-to-child transmission of HCV by polymerase chain reaction (PCR) and observed the course of the disease in infants. The subjects were 10 high-risk pregnant women with parenteral or sexually transmitted diseases who were hospitalized at AIDS centers. Blood was collected under aseptic conditions, cord blood was collected with a vascular catheter so as not to be contaminated with maternal blood. Plasma was tested with Ortho anti-HCV enzyme immunoassay (EIA) and repeated with Chiroa recombinant Western blotting assay (RIBA). In pregnant women near childbirth or childbirth test ALT, the baby at 4 to 6 months of age