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目的:探讨缺氧诱导因子-1α(HIF-1α)和热休克蛋白9Oα(HSP90α)在肝细胞癌中的表达及其与临床病理特征之间的关系。方法:采用免疫组织化学Envision二步法检测H HIF-1α和HSP90α蛋白在65例肝细胞癌和癌旁组织、20例正常肝组织中的表达,并分析其与肝细胞癌临床病理因素的关系。结果:HCC组织中HIF-1α和HSP90α阳性表达率均明显高于癌旁组织及正常肝脏组织,差异均具有统计学意义(P<0.01)。HIF-1α在HCC组织中的表达与肿瘤大小、临床分期、病理分级、有无淋巴结转移、有无门静脉癌栓密切相关(P<0.01),HSP90α在HCC组织中的表达与临床分期、病理分级、有无淋巴结转移、有无门静脉癌栓密切相关(P<0.01)。Spearman相关性检验分析提示HIF-1α和HSP90α表达阳性程度呈正相关(r=0.536,P<0.01)。结论:HIF-1α和HSP90α参与肝细胞癌的发生、发展,并起协同作用,可能作为判断肝细胞癌预后的指标。
Objective: To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and heat shock protein 9oα (HSP90α) in hepatocellular carcinoma (HCC) and its relationship with clinicopathological features. Methods: The expression of HIF-1α and HSP90α protein in 65 cases of hepatocellular carcinoma and adjacent non-cancerous tissues and 20 cases of normal liver tissues were detected by immunohistochemistry Envision two-step method. The relationship between the expression of HIF-1α and HSP90α and clinical and pathological factors of hepatocellular carcinoma . Results: The positive rates of HIF-1α and HSP90α in HCC tissues were significantly higher than those in paracancer tissues and normal liver tissues (P <0.01). The expression of HIF-1α in HCC tissues was closely related to tumor size, clinical stage, histological grade, lymph node metastasis and presence or absence of portal vein tumor thrombus (P <0.01). The expression of HSP90α in HCC tissues was correlated with clinical stage, pathological grade , With or without lymph node metastasis, with or without portal vein tumor thrombosis were closely related (P <0.01). Spearman correlation test showed that the positive expression of HIF-1α and HSP90α were positively correlated (r = 0.536, P <0.01). Conclusion: HIF-1α and HSP90α are involved in the occurrence, development and synergistic effect of hepatocellular carcinoma, which may be used as an index to judge the prognosis of hepatocellular carcinoma.