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在HCVRNA高度保守的5’末端非编码区域内选择合成内、外引物，建立了HCV的逆转录－套式PCR检测方法，并对血清标本中HCVRNA的提取方法进行比较。对23例肝病患者的检测结果显示，HCVRNA的阳性率为56．5％。与抗HCV抗体检测结果比较，抗HCV阳性患者中有81．5％HCVRNA阳性，而抗HCV阴性患者中仍有33．3％的HCVRNA阳性，表明PCR在HCV感染的早期诊断、传染性的确立及药物疗效考核等方面有重要作用。
The internal and external primers were synthesized in the highly conserved 5 ’end non-coding region of HCV RNA to establish a reverse transcription-nested PCR detection method for HCV, and the comparison of HCV RNA extraction methods in serum samples. The results of 23 patients with liver disease showed that the positive rate of HCVRNA was 56.5%. Compared with the results of anti-HCV antibody test, 81.5% HCV RNA was positive in anti-HCV positive patients, while 33.3% of HCV RNA was still positive in anti-HCV negative patients, indicating that PCR was used in the early diagnosis and infectivity of HCV infection And drug efficacy assessment plays an important role.