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目的检测大肠癌组织磷酸化丝氨酸/苏氨酸激酶(pAkt)、血管内皮生长因子(VEGF)的表达和微血管密度(MVD)及其临床意义。方法应用免疫组化SP法检测76例大肠癌的pAkt、VEGF和MVD。结果大肠癌pAkt和VEGF的阳性表达率分别为73.7%(56/76)和85.5%(65/76)。pAkt的阳性表达与肿瘤浸润深度、临床分期和淋巴结转移(P<0.05)及其MVD(P<0.01)显著相关。pAkt阳性组的MVD明显高于阴性组。VEGF的阳性表达与大肠癌的临床分期显著相关(P<0.05)。pAkt蛋白表达与VEGF蛋白表达密切相关(P<0.05)。结论pAkt和VEGF与大肠癌发生、发展和转移密切相关,提示阻断pI3K/Akt信号传导通路将对大肠癌的治疗提供新的靶点。
Objective To detect the expression of phosphorylated serine / threonine kinase (pAkt), vascular endothelial growth factor (VEGF) and microvessel density (MVD) in colorectal carcinoma and its clinical significance. Methods Immunohistochemical SP method was used to detect pAkt, VEGF and MVD in 76 cases of colorectal cancer. Results The positive rates of pAkt and VEGF in colorectal cancer were 73.7% (56/76) and 85.5% (65/76), respectively. The positive expression of pAkt was significantly correlated with the depth of invasion, clinical stage and lymph node metastasis (P <0.05) and MVD (P <0.01). The MVD in pAkt-positive group was significantly higher than that in the negative group. The positive expression of VEGF was significantly correlated with the clinical stage of colorectal cancer (P <0.05). The expression of pAkt protein was closely related to the expression of VEGF protein (P <0.05). Conclusion pAkt and VEGF are closely related to the occurrence, development and metastasis of colorectal cancer, suggesting that blocking the pI3K / Akt signaling pathway will provide a new target for the treatment of colorectal cancer.