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目的:建立肾癌原位肺转移动物模型,研究动物模型肾癌移植瘤和肺部转移瘤的生长情况,确定肾癌肺转移的初始时间。方法:人肾癌细胞株SN12-PM6种植于裸鼠右肾实质内,连续观察裸鼠肾脏肿瘤及肺部转移瘤的生长情况。所有采用标本10%甲醛溶液固定,常规石蜡包埋,切片HE染色后分析结果。结果:肾脏的成瘤率为100%(39/39),第5周开始观察到肿瘤的肺转移灶,肺部转移率50%(4/8),在随后3周其肺部转移率分别为75%(3/4),100%(8/8)和100%(7/7),肠道、肝脏、脾脏等脏器未发现明显的转移灶。结论:成功构建人肾细胞癌原位裸鼠肺转移动物模型,为肾癌特别是肾癌肺部转移提供了一个非常理想的研究模型。
OBJECTIVE: To establish an animal model of orthotopic lung metastasis of renal cell carcinoma and to study the growth of renal allograft and lung metastases in animal models and to determine the initial time of lung metastasis of renal cell carcinoma. Methods: The human renal cell carcinoma cell line SN12-PM6 was implanted in the right renal parenchyma of nude mice and the growth of the renal tumors and lung metastases in nude mice were observed continuously. All samples were fixed with 10% formalin, embedded in paraffin, sliced HE staining analysis results. Results: The rate of tumor formation in the kidney was 100% (39/39). Lung metastases were observed in the fifth week, and lung metastases were 50% (4/8) in the fifth week and lung metastases in the third week 75% (3/4), 100% (8/8) and 100% (7/7). No obvious metastasis was found in the intestine, liver, spleen and other organs. Conclusion: The in vivo model of lung metastasis of human renal cell carcinoma in nude mice was successfully constructed, which provided a very ideal model for the lung metastasis of renal cell carcinoma, especially of renal cell carcinoma.