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目的探讨生草乌及清蒸草乌的遗传毒性。方法应用鼠伤寒沙门菌体外回复突变试验(Ames)和彗星实验(Cometassay)对生草乌及清蒸草乌的遗传毒性进行研究。结果生草乌Ames试验中TA97、TA100、TA102菌株无论代谢活化或非代谢活化,皆为阳性,而TA98菌株则为阴性;清蒸草乌Ames试验为阴性。彗星实验在生草乌1.25,2.5,5.0mg/mL浓度下,尾长分别为(16.55±7.81),(23.68±8.32),(30.18±11.35)px,尾部DNA含量分别为(13.65±6.21),(20.55±8.55),(28.72±13.39)px2,与阴性对照组尾长(8.08±2.59)px、尾部DNA含量(6.33±4.16)px2比较,差异有统计学意义(P<0.01),且呈剂量-效应关系;而清蒸草乌各剂量组与阴性对照组比较差异无统计学意义。结论生草乌具有一定的遗传毒性,而清蒸草乌无遗传毒性,清蒸起到了较好的减毒效果。
Objective To investigate the genotoxicity of Shengcaowu and Qingcaowu. Methods The genotoxicity of Salvia miltiorrhiza Bge. And Avena sativa L. were determined by Salmonella typhimurium in vitro test (Ames) and Cometassay (Cometassay). Results In the Ames test, the TA97, TA100 and TA102 strains were both positive for metabolism or non-metabolic activation, while the TA98 strain was negative for the Ames test. The tail length of comet assay was (16.55 ± 7.81), (23.68 ± 8.32), (30.18 ± 11.35) px, and the tail DNA contents were (13.65 ± 6.21)% at the concentrations of 1.25, 2.5 and 5.0 mg / , (20.55 ± 8.55) and (28.72 ± 13.39) px2, respectively. The difference was significant (P <0.01) with tail length (8.08 ± 2.59) px and tail DNA content (6.33 ± 4.16) px2 The dose-effect relationship; and each dose of steamed grass and black control group was no significant difference between the negative control group. Conclusion Scutellaria has some genetic toxicity, but no genetic toxicity of steamed grass, steamed played a better attenuating effect.