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目的:研究五味子粗多糖提取物对复合病因诱导的大鼠肝纤维化的防治作用。方法:实验分为6组,即正常对照(生理盐水)、模型(生理盐水)、秋水仙碱(0.1mg·kg-1)和五味子粗多糖提取物高、中、低剂量(400、200、100mg·kg-1)组。腹腔注射CCl4复制大鼠肝纤维化模型;灌胃给药。检测大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的活性,肝匀浆丙二醛(MDA)和羟脯氨酸(HOP)的含量以及超氧化物岐化酶(SOD)的活性;形态学上观察肝组织病理学变化,用细胞图像分析系统(CIAS)对肝纤维化进行半定量。结果:与正常对照组比较,Massion染色显示模型组肝纤维化明显,有大量胶原纤维存在,肝小叶结构紊乱,血清ALT和AST显著升高,肝中MDA和HOP含量显著升高,SOD活性显著下降(P<0.01)。与模型组比较,各用药组大鼠血清ALT和AST显著降低;肝中MDA含量下降,SOD活性升高,具有显著性差异(P<0.01),并呈剂量依赖效应;肝HOP含量及胶原面积百分比显著下降(P<0.01)。结论:五味子多糖可减轻肝损害、肝脂质过氧化和肝纤维化的作用。
Objective: To study the prevention and treatment of schisandra chinensis polysaccharides extract on rat liver fibrosis induced by compound etiology. Methods: The experiment was divided into six groups: normal control (saline), model (saline), colchicine (0.1 mg · kg-1) and Schisandra chinensis polysaccharide extract at high, medium and low doses 100mg · kg-1) group. Intraperitoneal injection of CCl4 rat liver fibrosis model; intragastric administration. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA) and hydroxyproline (HOP) The activity of SOD was observed. Morphological changes of hepatic tissue were observed. Semiquantitative quantitation of hepatic fibrosis was performed by using cell image analysis system (CIAS). Results: Compared with the normal control group, Massion staining showed that the model group had obvious hepatic fibrosis with a large number of collagen fibers, hepatic lobule structure disorder, serum ALT and AST significantly increased, liver MDA and HOP content significantly increased, SOD activity significantly Decreased (P <0.01). Compared with the model group, serum ALT and AST of rats in each group were significantly decreased; MDA content in liver decreased, SOD activity increased significantly (P <0.01), and in a dose-dependent manner; liver HOP content and collagen area The percentage decreased significantly (P <0.01). Conclusion: Schisandra polysaccharide can reduce liver damage, liver lipid peroxidation and liver fibrosis.