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组蛋白修饰在植物发育和防御反应中发挥着重要的作用。为研究组蛋白去乙酰化酶基因HD1的基本特征及其在乙烯调控凤梨科植物开花过程中的生物学功能,通过筛选粉菠萝茎尖转录组测序数据并结合RACE技术分离得到AfHD1基因cDNA全长序列。该基因的开放阅读框长为1 548 bp,编码516个氨基酸,其理论分子量为58.28 kDa,等电点为5.23。由该基因编码的蛋白属于组蛋白去乙酰化酶RPD3/HDA1超家族成员,具有该家族特有的HDAC保守结构域,该蛋白与水稻和玉米中HD1蛋白的同源性均达到87%。实时荧光定量PCR分析表明,AfHD1基因的表达受乙烯诱导,处理后1 d的表达量与0 h相比,提高了4倍,推测组蛋白去乙酰化酶基因AfHD1可能与粉菠萝的乙烯信号反应有关。
Histone modification plays an important role in plant development and defense response. In order to study the basic characteristics of histone deacetylase (HD1) gene and its biological function during the flowering process of ethylene by regulating ethylene in pineapple, we screened the full-length cDNA of AfHD1 gene sequence. The open reading frame of this gene is 1 548 bp, encoding 516 amino acids. Its theoretical molecular weight is 58.28 kDa and its isoelectric point is 5.23. The protein encoded by this gene belongs to a member of the histone deacetylase RPD3 / HDA1 superfamily and has a unique HDAC conserved domain of this family, which has an identity of 87% with HD1 protein in rice and maize. Real-time PCR analysis showed that the expression of AfHD1 gene was induced by ethylene, and the expression level of AfHD1 increased by 4-fold on the 1st day after treatment compared with that on 0 h. It is speculated that the histone deacetylase gene AfHD1 may react with the ethylene signal of the powder pineapple related.