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收集8—26周人体胚胎,共22例,取胃底部及成人胃底,用生物素化凝集素GSAⅡ进行组化染色,光镜观察.GSAⅡ能特异性地与糖蛋白中的GlcNAc结合,在成人胃底腺,GSAⅡ与颈粘液细胞结合,局限于胃腺颈部,位于壁细胞之间,呈烧瓶状或柱状,DAB显色反应可见胞质中有棕色细颗粒.胃上皮细胞及胃底腺其它细胞呈阴性反应.12—16周,为原始胃腺泡期,GSAⅡ阳性细胞在腺泡底部,分布在体大、质多的原始壁细胞
A total of 22 human embryos were collected from 8 to 26 weeks, and the gastric fundus and adult gastric fundus were collected and stained with biotinylated lectin GSAⅡ.Gas spectroscopy showed that GSA Ⅱ could specifically bind to GlcNAc in glycoprotein, In adult gastric fundus, GSA Ⅱ binds to mucus cells in the neck and is confined to the neck of the stomach gland, located between the parietal cells in a flask-like or columnar shape. The DAB color reaction shows that there are brown fine granules in the cytoplasm. The other gland cells were negative reaction .12-16 weeks, the original gastric acinus, GSA Ⅱ positive cells in the bottom of the acinar, located in the body, the mass of primitive parietal cells