论文部分内容阅读
目的 :探讨 P1 6在喉癌组织中的表达及和喉癌发生发展的关系。方法 :采用免疫组化S- P法 ,对 56例喉癌组织 ,30例喉癌旁组织及 1 0例正常喉粘膜中 P1 6的表达进行检测 ,应用全自动图像分析仪对染色结果进行定量测定。结果 :喉癌组织中 P1 6的表达量明显低于癌旁组织及正常喉粘膜 ( P<0 .0 5) ,喉鳞状细胞癌 级明显低于 ～ 级 ( P<0 .0 5) ,颈淋巴结转移组明显低于无颈淋巴结转移组 ( P<0 .0 5)。结论 :P1 6在喉癌组织中的表达常为缺失 ,说明 P1 6作为抑癌基因在喉癌的发生中起作用。 P1 6的表达程度越低 ,分化越差 ,越易于浸润和转移 ,P1 6的检测在临床上可能作为估计预后的指标之一。
Objective: To investigate the expression of P1 6 in laryngeal carcinoma and the relationship with the occurrence and development of laryngeal carcinoma. Methods: Immunohistochemical S-P method was used to detect the expression of P1 6 in 56 cases of laryngeal carcinoma, 30 cases of laryngeal squamous cell carcinoma and 10 cases of normal laryngeal mucosa. The automatic image analyzer was used to quantify the staining results Determination. Results: The expression of P1 6 in laryngeal carcinoma tissues was significantly lower than that in paracancerous tissues and normal laryngeal mucosa (P <0.05). The laryngeal squamous cell carcinoma grade was significantly lower than that in laryngeal squamous cell carcinoma (P <0.05) Cervical lymph node metastasis was significantly lower than those without cervical lymph node metastasis (P <0.05). Conclusion: The expression of P1 6 in laryngeal carcinoma is often absent, which indicates that P1 6 plays a role in tumorigenesis of laryngeal carcinoma. The lower the expression level of P1 6, the worse the differentiation and the easier it is to infiltrate and metastasize. The detection of P1 6 may be one of the indicators of prognosis in clinic.