目的:观察重度创伤患者sCD14水平的变化与病情的关系。方法:ELISA法检测外周血单个核细胞体外培养上清液和外周血血浆中sCD14含量和五分类法检测外周血单核细胞数量和比率。结果:22例重度创伤患者手术24h后外周静脉血单个核细胞培养上清液中的sCD14水平(4.50ng/ml)较健康对照组(1.17ng/ml)明显升高(P<0.05),而手术后48h的sCD14有明显降低(1.15ng/ml),血浆中的sCD14在手术前较高(11.85ng/ml),同时外周血单核细胞数量和比率不同程度升高。结论:在重度创伤患者检测sCD14和外周血单核细胞的变化有一定的临床意义。 Objective: To observe the relationship between the changes of sCD14 and the severity of severe trauma patients. Methods: The levels of sCD14 in peripheral blood mononuclear cells supernatant and peripheral blood were detected by ELISA. The number and ratio of peripheral blood mononuclear cells were detected by five-category method. Results: The levels of sCD14 (4.50ng / ml) in peripheral venous blood mononuclear cell culture supernatants in 22 severe traumatic patients were significantly higher than those in healthy controls (1.17ng / ml) The sCD14 was significantly decreased (1.15ng / ml) 48h after surgery and the sCD14 in plasma was higher (11.85ng / ml) before surgery. The number and ratio of peripheral blood mononuclear cells increased to different degrees. Conclusion: The detection of sCD14 and peripheral blood mononuclear cells in patients with severe trauma has certain clinical significance.