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为了解新乡地区2011年肠道病毒71型(EV71)VP1基因特征及手足口病流行特点,采用荧光RT-PCR对临床诊断的粪便标本进行总肠道病毒(EV)、柯萨奇病毒A16(CA16)和EV71检测;选取10例EV71阳性标本进行VPl序列扩增并测序,所得序列进行同源性分析和构建系统发生树;对2011年新乡市手足口病疫情监测数据进行分析。结果显示,重症标本的EV71阳性率(73%)显著高于CA16阳性率(19%)(P<0.01);10株新乡EV71分离株的核苷酸及氨基酸差异分别为2.8%和0.9%,属于C4亚型的C4a簇;9株VP1区第170位氨基酸为A,1株为V;与近缘的C4a型代表株相比,新乡优势株的氨基酸变异一般发生在VP1第292位氨基酸(T→A);2011年新乡市共上报手足口临床诊断病例1118例,92%的发病年龄在3岁以下,发病高峰分别出现在4和12月份,提示一定要加强手足口病预防控制,寒冷天气尤其不能忽视。
In order to understand the characteristics of VP1 gene of EV71 and the epidemic characteristics of hand-foot-mouth disease in 2011 in Xinxiang area, the stool samples of clinical diagnosis were collected for total enterovirus (EV), Coxsackievirus A16 CA16) and EV71. Ten EV71 positive samples were selected for amplification and sequencing of VP1 sequence. The homology analysis and phylogenetic tree analysis of the obtained sequences were carried out. The monitoring data of HFMD in Xinxiang City in 2011 were analyzed. The results showed that the EV71 positive rate (73%) in severe cases was significantly higher than that of CA16 (19%) (P <0.01). The nucleotide and amino acid differences of 10 EV71 strains in Xinxiang were 2.8% and 0.9% Which belongs to the C4a cluster of the C4 subtype. The amino acid sequence of the 170th VP1 region of the 9 strains is A and 1 is V. Compared with the C4a-type representative strains, the amino acid variation of Xinxiang dominant strain generally occurs at amino acid 292 of VP1 T → A). In 2011, Xinxiang City reported 1118 cases of hand, foot and mouth clinical diagnosis, 92% of the patients were under 3 years of age and the peak incidence occurred in April and December, respectively, suggesting that prevention and control of HFMD and cold In particular, the weather can not be ignored.