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本文用染色体C分带、同功酶和RAPD技术分析了来自不同地理分布的新麦草不同材料的单株,观察其c带的多态性,即同源染色体的配对,相同材料的不同个体之间,同一地区不同材料之间,以及不同来源的材料之间的多态性。10个同功酶揭示了14个假定标记位点,其中11个显示了多态性(平均66.2％,每个位点具2.6个等位基因)。大量的等位变化发生在多数的多态位点上。在所用试材中有近90％的等位变化。在优化条件下,200个测试引物中有55.5％的材料间产生了多态性的RAPDs带型。在同一材料中和不同材料间分别有55.7％和47.8％的扩增片断是多态的。C—带、同功酶和RAPD分析结果是互相确证而高度一致的,为新麦草的遗传变异提供了模式。 新麦草具有抗旱、耐盐碱和抗黄矮病的特性,可以在小麦育种工作上加以利用。
In this paper, chromosomes C zoning, isozymes and RAPD technology from different geographical distribution of different materials of new wheatgrass single plant, observe the c-band polymorphism, that is homologous chromosome pairing, the same material of different individuals Between different materials in the same area, and between different sources of materials. Ten isozymes revealed 14 putative marker sites, of which 11 showed polymorphism (average 66.2% with 2.6 alleles per locus). A large number of allelic changes occur in the majority of polymorphic sites. Almost 90% of the samples used in the changes in the bit. Under optimized conditions, 55.5% of the 200 test primers generated polymorphic RAPDs. In the same material and between different materials, 55.7% and 47.8% of the amplified fragments were polymorphic. C-banding, isozymes and RAPD analyzes are highly validated and highly consistent with each other, providing a model for genetic variation in P. triticina. Wheatgrass has the characteristics of drought resistance, salt tolerance and resistance to yellowish dwarfism and can be utilized in wheat breeding.