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目的:研究宫颈癌siha细胞在体外不同培养条件下细胞形态学的改变。方法:体外培养人宫颈癌siha细胞,以终浓度为1、5、10、15μmol/L的溶血磷脂酸和终浓度为5、10、20、40 mg/L的多西环素分别干预,并设不加任何药物作为空白对照组。各组均于干预后3、5、7天行过典酸雪夫反应(PAS)染色,用倒置显微镜观察各组宫颈癌细胞的形态变化。结果:溶血磷脂酸组siha细胞随着溶血磷脂酸浓度升高及作用时间的延长,细胞增殖能力增强,细胞异形性及可塑性增加,PAS染色阳性物质生成增多并最终形成环状结构的血管拟态;而多西环素处理组随着多西环素浓度增加,细胞增殖明显受到抑制,细胞核固缩、碎裂,细胞破坏。结论:宫颈癌siha细胞的增殖能力与细胞形态学的改变密切相关,溶血磷脂酸促进siha细胞增殖及血管生成拟态的形成,而多西环素则具有相反的抑制作用,且两者作用均有浓度及时间依赖性。
Objective: To study the morphological changes of siha cells in vitro under different culture conditions. Methods: Human cervical cancer siha cells were cultured in vitro. The cells were treated with lysophosphatidic acid at final concentrations of 1, 5, 10 and 15 μmol / L and doxycycline at final concentration of 5, 10, 20 and 40 mg / Set no drugs as a blank control group. All groups were stained with PAS by 3,5,7 days after the intervention, and morphological changes of cervical cancer cells in each group were observed by inverted microscope. Results: With the increase of lysophosphatidic acid concentration and prolongation of action time, the ability of cell proliferation and cell atypia and plasticity increased, the number of PAS staining positive substances increased and the vascularization of vasculature finally formed. The doxycycline treatment group with increasing concentrations of doxycycline, cell proliferation was significantly inhibited, nuclear pyknosis, fragmentation, cell destruction. CONCLUSION: The proliferation of siha cells in cervical cancer is closely related to the changes of cell morphology. Lysophosphatidic acid can promote the proliferation of siha cells and the formation of angiogenic mimicry, while doxycycline has the opposite inhibitory effect Concentration and time-dependent.