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以辽单527、辽单青贮178、辽原一号3个玉米品种为试验材料,通过6个浓度的秋水仙素溶液处理,进行染色体加倍,采用流式细胞仪检测加倍效果。结果表明,当秋水仙素浓度达0.1%时,3个品种加倍率均达到最高值,辽单527的加倍效果最好,最高可达36.3%。利用流式细胞仪能够快速准确检测染色体倍性,OTTO法制备悬浮液的检测效果较好。
Liao Dan 527, Liao Dan silage 178, Liaoyuan 1 three maize varieties as tested materials, through the concentration of colchicine solution 6, the chromosome doubling, the use of flow cytometry double effect. The results showed that when the concentration of colchicine reached 0.1%, the doubling rates of all three cultivars reached the highest value, and Liao Dian 527 had the best double effect, up to 36.3%. The use of flow cytometry can quickly and accurately detect chromosome ploidy, OTTO prepared suspension better detection effect.