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用Hela细胞粘附试验或肠聚集性大肠杆菌(E(Agg)EC)探针对E(Agg)EC进行鉴定,DNA探针的敏感度为41%,特异度为91%;用E(Agg)EC探针鉴定艾希氏大肠杆菌(E·Coli)的感染率,广州病例组为24%,对照组为31%(P=0.187),泰国病例组为11%,对照组为4%(P=0.014)。Hela细胞试验鉴定的E(Agg)EC感染率,广州病例组为11%,对照组为10%(P=0.983),曼谷病例组为5%,对照组9%。聚集性模式中粘附Hela细胞和用E(Agg)EC探针杂交的E·Coli,有27个O:H血清型及无血清型与腹泻有关。用Hela细胞试验或用E(Agg)EC探针杂交作鉴定的E(Agg)EC与广州儿童的腹泻病无关。腹泻儿童中在未发现其他肠道病原菌的情况下,E(Agg)EC可引起腹泻。
E (Agg) EC was identified using the Hela cell adhesion assay or enterotactic E.coli (E (Agg) EC) DNA probe with 41% sensitivity and 91% specificity; E ) EC probe was used to identify the infection rate of E.coli, 24% in Guangzhou case group, 31% in control group (P = 0.187), 11% in Thai case group and 4 in control group % (P = 0.014). The infection rate of E (Agg) EC identified by Hela cell assay was 11% in Guangzhou case group and 10% in control group (P = 0.983), 5% in Bangkok case group and 9% in control group. There are 27 O: H serogroups and serogroups associated with diarrhea in aggregated HeLa cells and E. Coli hybridized with E (Agg) EC probes. E (Agg) EC identified using Hela cell assays or hybridized with E (Agg) EC probes was not associated with diarrhea in children in Guangzhou. E (Agg) EC can cause diarrhea in children with diarrhea without other enteric pathogens.