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实验以培养的SD大鼠胸主动脉平滑肌细胞为模型，用四唑盐比色试验（MTT）法及3H－TdR掺入技术动态观察发现亮氨酸脑啡肽（L－ENK，10~5～10-4mol／L）可显著抑制血管平滑肌细胞（VSMCs）的增殖及DNA的合成，阿片受体阻断剂纳洛酮（NAL，10-8～10-5mol／L）对VSMCs的增殖及DNA的合成无影响，但NAL（10-5mol／L）可拮抗亮氨酸脑啡肽对VSMCs增殖的抑制作用。
In the present study, cultured rat thoracic aorta smooth muscle cells were used as a model, and leukotriene enkephalin (L-ENK, 10-5) was dynamically observed by tetrazolium colorimetric assay (MTT) and 3H-TdR incorporation ~ 10-4mol / L) could significantly inhibit the proliferation and DNA synthesis of vascular smooth muscle cells (VSMCs) and the proliferation of VSMCs induced by naloxone (NAL, 10-8-10-5 mol / L) DNA synthesis had no effect, but NAL (10-5mol / L) could antagonize leucine enkephalin on the proliferation of VSMCs inhibition.