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目的:采用病例-对照研究,探讨人类白细胞抗原(HLA)-G上游调控区-725单核苷酸多态性与子痫前期的发病是否存在关联。方法:对孕晚期重度子痫前期患者40例、正常足月妊娠妇女41例,提取全血基因组DNA,特异引物巢式聚合酶链反应扩增5’上游调控区,并进行基因测序,对-725位点基因型及等位基因频率进行χ2检验。结果:重度子痫前期组人类白细胞抗原-G基因-725单核苷酸多态性基因型频率GG、GC、CC分别为0%、7.5%、92.5%,正常对照组分别为0%、7.3%、92.7%,两组比较,差异无统计学意义(P>0.05)。重度子痫前期组该位点等位基因频率G、C分别为3.8%、96.2%,正常对照组分别为3.7%、96.3%,两组比较差异无统计学意义(P>0.05)。结论:HLA-G基因-725单核苷酸多态性与中国河南汉族人群重度子痫前期的发病可能没有直接相关。
OBJECTIVE: To investigate whether there is a correlation between -725 single nucleotide polymorphism (SNP) of human leukocyte antigen (HLA) -G upstream and preeclampsia using a case-control study. Methods: Forty pregnant women with severe preeclampsia in the third trimester of pregnancy and 41 normal pregnant women were enrolled. Genomic DNA was extracted from whole blood and the 5 ’upstream regulatory region was amplified by nested polymerase chain reaction (PCR) 725 locus genotypes and allele frequencies for χ2 test. Results: The frequency of GG, GC and CC genotypes in patients with severe preeclampsia was 0%, 7.5% and 92.5%, respectively, while that in the normal control group was 0% and 7.3% respectively %, 92.7% respectively. There was no significant difference between the two groups (P> 0.05). The frequencies of allele G and C in the severe preeclampsia group were 3.8% and 96.2% respectively, while those in the normal control group were 3.7% and 96.3% respectively. There was no significant difference between the two groups (P> 0.05). Conclusion: The single nucleotide polymorphism of HLA-G-725 may not be directly related to the occurrence of severe preeclampsia in Han nationality in Henan Province of China.