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甘蓝型黄籽油菜具有低纤维、高蛋白及高含油量的优点, 因而已成为广大油菜育种工作者研究的重点之一。利用甘蓝型黑籽品系油研 2 号作父本,甘蓝型黄籽品系 GH06为母本,获得 132 个单株的 F2群体;以 AFLP 和 SSR 为主要分析方法,构建了包括 164 个标记的甘蓝型油菜遗传连锁图谱,其中包括 125 个 AFLP 标记、37 个 SSR 标记及一个 RAPD 和一个 SCAR 标记,分布在 19 个连锁群上,覆盖油菜基因组 2 549.8 cM,标记间平均距离 15.55 cM。利用多区间作图法,对种皮色泽 QTL 进行分析,在第 5 及第 19 连锁群上各检测到一个 QTL 位点,分别解释表型变异 46%及 30.9%。
Yellow-seeded rapeseed has the advantages of low fiber, high protein and high oil content, and has become one of the focuses of the breeding workers in the rapeseed. The F2 population of 132 individuals was obtained from the male parent of Brassica campestris yaoyan 2 with the yellow cabbage type Yanou 2 and yellow seed of the blue cabbage type GH06. AFLP and SSR were used as the main analysis methods to construct the cabbage including 164 markers Rape genetic linkage map, including 125 AFLP markers, 37 SSR markers and a RAPD and a SCAR markers, distributed in 19 linkage groups, covering rape genome of 2 549.8 cM, the average distance between markers 15.55 cM. The QTLs of seed coat color were analyzed by using the multi-interval mapping method. A QTL locus was detected on the 5th and 19th linkage groups, which explained 46% and 30.9% of the phenotypic variation respectively.