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目的 探讨ToRCH系列感染与异常妊娠结局的关系 ,为做好优生优育提供有价值的实验依据。方法 采用间接ELISA对 45例有异常妊娠结局妇女作ToRCH 4种病原体抗体检测 ,并对部分IgM (或IgG)阳性者作了PCR检测。对其中的 2 1例与ToRCH感染有关的异常妊娠结局胚胎组织块作了PCR检测和对 10例异常新生儿作了母子配对血清学诊断。结果 45例异常妊娠结局妇女中ToX、RubV、CMV、HSV的IgM阳性率分别为 2 0 %、5 1%、44 4%和2 9 7% ,并发现同一病例有 2种或 2种以上病原体同时感染的情况 ,血清PCR结果与之基本一致。 2 1例异常妊娠结局者 (流产、早产、死胎、先天缺陷 ) ,其胚胎组织中 (人流混合物、死胎脑组织块 )PCR检测均有ToRCH系列中的 1种、2种或 2种以上病原体感染 ,与上述ELISA结果吻合。 10对新生儿脑炎血清与对应母亲血清抗体检测表明 ,垂直传播率ToX与CMV分别为 5 0 %和 40 %。结论 妇女ToRCH系列感染是造成异常妊娠结局的重要原因之一 ,必须进行检测和有效控制
Objective To investigate the relationship between ToRCH infection and abnormal pregnancy outcomes and to provide valuable experimental evidences for prenatal and postnatal care. Methods 45 cases of women with abnormal pregnancy outcome were tested for indirect immunosorbent assay (ELISA) against ToRCH 4 pathogens and PCR was performed on some IgM (or IgG) positive individuals. PCR was performed on 21 fetuses with abnormal pregnancy outcome associated with ToRCH infection and maternal-paired serological diagnosis of 10 abnormal newborns. Results The positive rates of IgM in ToX, RubV, CMV and HSV in 45 patients with abnormal pregnancy outcome were 20%, 51%, 444% and 297%, respectively, and found that there were 2 or more pathogens in the same case At the same time infection, serum PCR results and basically the same. Twenty-one patients with abnormal pregnancy outcome (miscarriage, premature birth, stillbirth, congenital defect) and 1, 2 or more pathogen infections in ToRCH series were detected by PCR in embryonic tissues (human flow mixture, fetal brain tissue) , Consistent with the above ELISA results. 10 pairs of neonatal encephalitis serum and corresponding maternal serum antibody test showed that the vertical transmission rate ToX and CMV were 50% and 40%. Conclusion The ToRCH infection in women is one of the important causes of abnormal pregnancy outcome and must be tested and effectively controlled