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制备血吸虫病单克隆抗体诊断试剂盒,将其用于Dot—ELISA,分别检测江苏、安徽流行区急性血吸虫病患者及四川、江西、安徽流行区慢性血吸虫病患者血清中循环抗原。结果不同流行区Dot—ELISA反应的平均“+”值、阳性率和抗原滴度的几何均数不同,循环抗原水平可以反映流行区的疫情。另将Dot—ELISA、ELISA检测四川慢性血吸虫病人治前及治后半年、1年的血清循环抗原及抗体,结果循环抗原在病人治疗后很快下降,治后1年77.8％(35/45)病人的循环抗原转为阴性。循环抗体在病人治疗后也逐渐下降,但速度甚为缓慢。治疗后1年,仅21.7％(10/46)病人转为阴性。上述结果表明,循环抗原能更直接、更真实地反映防治效果。
Monoclonal antibody diagnostic kit for schistosomiasis was prepared and used for Dot-ELISA to detect circulating antigens in serum of patients with acute schistosomiasis in endemic areas of Anhui Province and Anhui Province, and in patients with chronic schistosomiasis in Sichuan, Jiangxi and Anhui Provinces. Results The average “+” values, positive rates and antigen titers of Dot-ELISA in different endemic areas were different. The circulating antigen level could reflect the epidemic situation. In addition, Dot-ELISA and ELISA were used to detect serum circulating antigens and antibodies in patients with chronic schistosomiasis before and after treatment for half a year and one year after treatment. The results showed that the circulating antigens rapidly decreased after treatment, with 77.8% (35/45) The patient’s circulating antigen turned negative. Circulating antibodies also gradually decreased after treatment, but at a very slow rate. One year after treatment, only 21.7% (10/46) patients turned negative. The above results show that the circulating antigen can more directly and more truly reflect the control effect.