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树突状细胞(dendritic cells,DC)是一种抗原递呈细胞,其最显著的特点是能有效刺激静息的T淋巴细胞,诱发初次免疫应答.DC来源于骨髓细胞,在正常组织中含量很少,在体外可用GM-CSF联合IL-4能从CD14~+单个核细胞培养出DC,细胞因子对DC的分化成熟和抗原递呈功能具有重要的调节作用.我们在体外成功的建立了16例肿瘤患者的自体DC细胞.血标本的来源为中晚期消化道肿瘤患者,年龄25～60岁,平均年龄52岁,其中男性9例,女性7例.实验方法:肿瘤患者外周血经CS-3000血细胞分离机采集,经Ficol淋巴细胞分层液密度梯度离心分离获得单个核细抱,计数
Dendritic cells (DCs) are antigen-presenting cells. Their most prominent feature is their ability to stimulate resting T lymphocytes and induce a primary immune response. DCs are derived from bone marrow cells and are present in normal tissue. Rarely, in vitro, GM-CSF and IL-4 can be used to culture DCs from CD14~+ mononuclear cells. Cytokines play an important role in the regulation of DC maturation and antigen-presenting function. We have successfully established in vitro. 16 autologous DC cells from tumor patients. Blood samples were obtained from patients with advanced digestive tract tumors, aged 25 to 60 years, mean age 52 years, including 9 males and 7 females. Experimental method: Peripheral blood of patients with cancer -3000 blood cell separator, collected by Ficol lymphocyte stratification liquid density gradient centrifugation to obtain a single nuclear fine hold, count