论文部分内容阅读
灰葡萄孢产生功能上类似附着胞的侵染垫来侵染寄主植物,但目前对于侵染垫形成的分子机制尚不明确。本文利用高通量测序技术对菌株CanBC-1(弱毒,不形成侵染垫)和CanBC-1c-66(强毒,正常形成侵染垫)进行了转录水平比较。结果表明:在菌株CanBC-1中共检测到2 333个差异表达基因(与菌株CanBC-1c-66相比较),其中1 425个基因表达上调,908个基因表达下调。对差异表达基因进行功能注释(GO)分析发现,在细胞组分(Cellular component)中细胞(Cell)和细胞部分(Cell part)这2个亚类所占比例较大,在分子功能(Molecular function)中结合活性(Binding)和催化活性(Catalytic)这2个亚类所占比例较大。这些结果暗示差异基因主要参与细胞、代谢和发育等生物学过程。筛选得到12个与真菌致病相关的基因,其中BC1G_03994和BC1G_01012与稻瘟病菌侵染相关的classⅡ疏水蛋白基因Mhp1同源。RT-PCR检测发现这2个基因在弱毒菌株CanBC-1中表达下调,同时该菌株疏水性降低,推测它们可能参与灰葡萄孢侵染垫形成。这些差异基因的获得为揭示灰葡萄孢侵染形成的分子机制奠定了基础。
Botrytis cinerea produces fungi that are functionally attached to attachment cells to infect host plants, but the molecular mechanisms for the formation of infection pads are not yet clear. In this paper, high-throughput sequencing was used to compare the transcriptional levels of CanBC-1 (attenuated, non-infiltrating pad) and CanBC-1c-66 (virulent, normally infiltrating pad). The results showed that a total of 2 333 differentially expressed genes were detected in CanBC-1 strain (compared with CanBC-1c-66 strain), of which 1 425 genes were up-regulated and 908 genes were down-regulated. A functional annotation (GO) analysis of the differentially expressed genes revealed that the two subtypes of Cell and Cell part account for a large proportion in the cellular component, ) In the binding activity (Binding) and catalytic (Catalytic) these two subclasses accounted for a larger proportion. These results suggest that differential genes are mainly involved in biological processes such as cell, metabolism and development. Twelve genes related to fungal pathogenicity were screened, of which BC1G_03994 and BC1G_01012 were homologous to the class II hydrophobin gene Mhp1 related to infection by Magnaporthe grisea. The RT-PCR results showed that the two genes were down-regulated in the attenuated CanBC-1 strain. At the same time, the hydrophobicity of the two strains was decreased, suggesting that they may be involved in the formation of Botrytis cinerea invasion pad. The availability of these differential genes laid the foundation for revealing the molecular mechanism of Botrytis cinerea infection.