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雄性大鼠２１只（体重２００～２５０ｇ），随机分为Ａ、Ｂ、Ｃ三组，氨基甲酸乙酯（１ｇ／ｋｇ）腹腔麻醉，双针记录电极置于右侧股二头肌以记录肌电，双侧腋窝皮下埋藏板状电极以引导心电信号。肌电信号及心电信号，经生物信号处理系统（ＳＭＵＰ－ＰＣ）Ｚ自动记录心电幅值、频率及屈肌反射阈值、潜伏期，并进行定量分析处理。尾静脉注射蝎毒纯化因子Ⅱ（ＡＦＳＶＣ－Ⅱ）的剂量，Ａ组为４３μｇ／ｋｇ，Ｂ组为６５μｇ／ｋｇ，Ｃ组为１３０μｇ／ｋｇ，注入容量均为０．３ｍｌ。ＡＦＳＶＣⅡ注射前及注射后（每隔１０ｍｉｎ测定１次，８０ｍｉｎ停止观察）分别对上述各项生理指标进行测定。结果：ＡＦＳＶＣ－Ⅱ尾静脉注射４３～６５μｇ／ｋｇ对心电幅值没有显著影响，但注射１３０μｇ／ｋｇ１０～３０ｍｉｎ，可明显减低心电幅值；ＡＦＳＶＣ－Ⅱ尾静脉注射４３～６５μｇ／ｋｇ对屈肌反射没有显著影响，但注射１３０μｇ／ｋｇ１０～３０ｍｉｎ，可明显减低神经肌肉的兴奋性及减慢神经传导的速度。提示：蝎毒可通过抑制Ｎａ＋通道，从而明显降低神经肌肉的兴奋性。
Twenty-one male rats weighing 200-250 g were randomly divided into three groups: A, B and C, and urethane (1 g / kg) was given intraperitoneally. The double-needle recording electrode was placed on the right biceps femoris muscle Electric, bilateral axillary buried plate electrodes to guide the ECG signal. Electromyography and electrocardiogram (ECG) signal, the ECG amplitude, frequency and flexor reflex threshold and latency were automatically recorded by the SMUP-PC Z and quantitative analysis was performed. The dose of AFSVC-Ⅱ was injected into tail vein at a dose of 43 μg / kg in group A, 65 μg / kg in group B, and 130 μg / kg in group C, with an infusion volume of 0.3 ml. AFSVC Ⅱ before injection and after injection (every 10min measured 1, 80min stop observation) were measured for each of the above physiological indicators. Results: The tail vein injection of AFSVC-Ⅱ at the dose of 43 ~ 65μg / kg had no significant effect on the amplitude of electrocardiogram. However, the injection of 130μg / kg for 10 ~ 30min significantly reduced the amplitude of ECG; Flexor reflex had no significant effect, but the injection of 130μg / kg10 ~ 30min, can significantly reduce the excitability of neuromuscular and slow nerve conduction velocity. Tip: Scorpion venom can inhibit Na + channels, thereby significantly reducing neuromuscular excitability.