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以胡萝卜白色野生资源‘松滋野生’和欧洲橘色栽培品种‘Amsterdam’及其回交重组自交系(BILs)中的5个不同根色株系为试材,研究其根和叶中α–胡萝卜素、β–胡萝卜素合成途径分支点上LCYE、LCYB1、CHXE、CHXB1基因转录表达与类胡萝卜素含量之间的关系。c DNA测序结果表明,松滋野生和Amsterdam之间存在多个SNP变异位点。类胡萝卜素测定发现,Amsterdam叶中α–胡萝卜素含量(87.3μg·g-1)显著高于松滋野生(2.8μg·g-1),而其β–胡萝卜素含量(122.7μg·g-1)显著低于松滋野生(237.9μg·g-1)。q RT-PCR结果显示,LCYE、LCYB1、CHXE和CHXB1在不同材料的根和叶中均表达,其中LCYE与根中α–胡萝卜素、β–胡萝卜素及总类胡萝卜素含量之间呈显著正相关;与叶中叶黄素和总类胡萝卜素含量之间呈显著负相关,说明LCYE对类胡萝卜素积累起着关键性作用。
Five different root color lines of ’White Pine’, a white wild resource of carrot, and ’Orange’, a European orange cultivar, and backcross recombinant inbred lines (BILs) were used as materials to study the effect of α Relationship between the transcriptional expression of LCYE, LCYB1, CHXE, CHXB1 gene and carotenoid content at the branching point of carotene and β-carotene synthesis pathway. c DNA sequencing results showed that there are multiple SNP variation sites between Songzi wild and Amsterdam. The carotenoid assay showed that the α-carotene content in Amsterdam leaves was significantly higher than that in the wild (2.8 μg · g -1) and that of β-carotene (122.7 μg · g -1) 1) was significantly lower than that of wild pine (237.9μg · g-1). q RT-PCR results showed that LCYE, LCYB1, CHXE and CHXB1 were expressed in roots and leaves of different materials, among which LCYE was positively correlated with α-carotene, β-carotene and total carotenoid in root And negatively correlated with the content of lutein and total carotenoids in leaves, indicating that LCYE plays a key role in the accumulation of carotenoids.