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目的 :探索拉伸应变与血管平滑肌细胞 (VSMC)形态变化的关系 ,了解VSMC在血管壁对应力适应性改建中的响应。方法 :运用自行研制的基底膜伸张装置实验系统 ,通过液压工作方式对培养于硅胶膜上的VSMC施以不同强度或不同时间的应变影响 ,模拟生理应力微环境中VSMC受到的二维拉伸应变 ,结合显微形态观察 ,计算机图像处理 ,了解拉伸应变与VSMC形态变化的定量关系。结果 :①加载 3min～ 6h内VSMC铺展进行性增加 ,且较对照组快 ,6～ 2 4h铺展面积缩小 ,细胞长 /短径比与加载时间正相关 ;②加载培养可影响VSMC的排布 ,且细胞排布方式与加载时间有关 ;③融合生长的细胞较非融合生长的细胞加载后出现明显的舒 缩活动。结论 :①应变对VSMC形态变化呈双向影响 ;②适宜的应变刺激对VSMC表型维持有积极影响。
OBJECTIVE: To investigate the relationship between tensile strain and morphological changes of vascular smooth muscle cells (VSMCs), and to understand the response of VSMC to the adaptation of vascular wall to stress. Methods: The self-developed basement membrane stretching device experimental system was used to simulate the two-dimensional tensile strain of VSMC in physiological stress microenvironment by means of hydraulic working on the VSMCs cultured on silica gel membrane with different intensities or different time. , Combined with microscopic morphological observation, computer image processing, to understand the quantitative relationship between tensile strain and morphological changes of VSMC. Results: (1) VSMC spreading increased within 3min ~ 6h, and it was faster than control group. The spreading area was reduced in 6 ~ 24h and the cell length / short diameter ratio was positively correlated with loading time. (2) The loading culture could affect the arrangement of VSMC, And the arrangement of cells was related to the loading time. (3) The fused cells grew significantly more than the non-fused cells. Conclusion: ① Strain has a bidirectional effect on the morphology of VSMCs; ② Appropriate strain stimulation has a positive effect on VSMC phenotype maintenance.