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目的探讨线粒体ＤＮＡ突变与氨基糖甙类抗生素致聋的关系。方法应用ＰＣＲ－ＢｓｍＡＩ酶切及ＰＣＲ产物直接银染测序，对４８例氨基糖甙类抗生素致聋散发患者外周血标本的线粒体ＤＮＡ进行分析。结果发现６例患者线粒体ＤＮＡ１２ＳｒＲＮＡ基因核苷酸第１５５５位点发生了Ａ→Ｇ突变。结论氨基糖甙类抗生素致聋有明显的个体差异，带有１５５５Ｇ的个体对氨基糖甙类抗生素的耳毒作用有高度易感性。
Objective To investigate the relationship between mitochondrial DNA mutations and aminoglycoside-induced deafness. Methods The mitochondrial DNA of peripheral blood samples of 48 patients with aminoglycoside-induced deafness were analyzed by PCR-BsmAI digestion and direct silver sequencing of PCR products. The results showed that 6 cases of mitochondrial DNA 12SrRNA nucleotide nucleotide 1555 A → G mutation occurred. Conclusion Aminoglycoside antibiotics deaf have obvious individual differences. Individuals with 1555G have a high susceptibility to ototoxicity of aminoglycoside antibiotics.