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人类疱疹病毒 7 型(HHV-7)的感染依赖于包膜糖蛋白在病毒生命周期的多个阶段发挥功能. 这些蛋白质可以介导病毒吸附,病毒包膜和宿主细胞膜融合以及病毒在细胞间的接触传播. 将表达 HHV-7 糖蛋白的 293T 细胞与 HHV-7 易感的SupT1 细胞共培养,检测虫荧光素酶报告基因的表达,以鉴定介导膜融合的 HHV-7 糖蛋白. 研究发现,HHV-7 糖蛋白 gB、gH、gL、gO 能介导 293T 细胞与 SupT1 细胞的融合,且融合可被抗 CD4 单抗所抑制. 结果表明,糖蛋白 gB、gH、gL、gO对于 HHV-7 引发的膜融合是必需的,其中某个蛋白质或所形成的蛋白质复合物可能是 CD4 的配体.
Infection with human herpesvirus type 7 (HHV-7) depends on the role of envelope glycoproteins in multiple stages of the virus's life cycle, and these proteins can mediate viral adsorption, viral envelope and host cell membrane fusion, and virus intercellular Contact propagation.The 293T cells expressing HHV-7 glycoprotein were co-cultured with HHV-7-susceptible SupT1 cells and the expression of the luciferase reporter gene was examined for the identification of HHV-7 glycoprotein that mediates membrane fusion. GH, gL, gO of HHV-7 glycoprotein could mediate the fusion of 293T cells with SupT1 cells, and the fusion could be inhibited by anti-CD4 mAb.The results showed that the glycoproteins gB, gH, gL, 7-initiated membrane fusion is required, where a certain protein or formed protein complex may be a CD4 ligand.