论文部分内容阅读
实验用Ｗｉｓｔａｒ大鼠３５只，体重２１０ｇ。异丙肾（ＩＳＰ）组２８只，皮下注射ＩＳＰ５．０ｍｇ／ｋｇ，注射后４、１２、２４和４８小时分别处死７只大鼠，随同处死对照组７只。光镜观察心肌坏死部位和程度；电镜观察心肌超微结构变化；用体视学方法定量分析心肌线粒体形态变化。结果表明：心肌线粒体Ｖｖ和Ｖ值升高，与对照组比较差异显著（Ｐ＜０．０１），ＮＡ和Ｎｖ值变化与对照组比较无显著差异（Ｐ＞０．０５）。实验表明线粒体Ｖｖ和Ｖ植升高主要与线粒体肿胀有关，与线粒体的集聚和增生无关；线粒体的损伤破坏与心肌坏死程度密切相关；并提示注射ＩＳＰ后４８小时是制作大鼠心肌坏死模型的最佳时间。
35 Wistar rats were used in experiment, weighing 210 g. 28 rats in the isoproterenol (ISP) group and 5 rats in the subcutaneous injection of ISP5.0 mg / kg. Seven rats were sacrificed at 4, 12, 24 and 48 hours after injection respectively. Seven rats in the control group were sacrificed. The location and extent of myocardial necrosis were observed with light microscope. The ultrastructure of myocardium was observed by electron microscope. The morphological changes of myocardial mitochondria were quantitatively analyzed by stereological methods. The results showed that the Vv and V of myocardial mitochondria increased significantly compared with the control group (P <0.01). The changes of NA and Nv were not significantly different from those of the control group (P> 0.05). Experiments show that mitochondrial Vv and V increased plant mainly associated with mitochondrial swelling, and mitochondria aggregation and proliferation has nothing to do; mitochondrial damage and myocardial necrosis is closely related to the degree; and prompted 48 hours after injection of ISP is the most important model of rat myocardial necrosis Good time.