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目的:探讨急性非厌氧菌性肺炎患儿感染和治疗过程中免疫功能的变化。方法:选取急性非厌氧菌性肺炎患儿41例为观察组,根据细菌培养结果选择敏感抗生素和匹多莫得进行治疗,分别于治疗前后抽取患者空腹静脉血,以流式细胞术检测CD3+T细胞、CD4+T细胞、CD8+T细胞及CD16+CD56+NK细胞的计数,以ELISA试剂盒检测免疫球蛋白Ig A、Ig M、Ig G及炎症因子TNF-α、IL-6、IL-8的含量。并以41例同期健康志愿者为对照组,进行对比研究。结果:观察组患者治疗前CD3+T细胞、CD4+T细胞、CD4+/CD8+及CD16+CD56+NK细胞计数及免疫球蛋白Ig A、Ig M、Ig G含量显著低于对照组(P<0.05或P<0.001),治疗后明显升高(P<0.05或P<0.001);观察组患者治疗前炎症因子TNF-α、IL-6、IL-8含量显著高于对照组(P<0.001),治疗后明显降低(P<0.05或P<0.001)。结论:非厌氧菌性肺炎患儿细胞免疫和体液免疫功能低下,且存在循环系统炎症反应,在抗生素治疗的基础上进行免疫治疗对于改善病情有积极作用。
Objective: To investigate the changes of immune function in children with acute non-anaerobic pneumonia during infection and treatment. Methods: A total of 41 children with acute non-anaerobic pneumonia were selected as the observation group. The patients were treated with selective antibiotics and pidoterone according to the results of bacterial culture. Fasting venous blood samples were collected before and after treatment. Flow cytometry was used to detect CD3 T cells, CD4 + T cells, CD8 + T cells and CD16 + CD56 + NK cells were measured by ELISA kit. Immunoglobulin Ig A, Ig M, Ig G and inflammatory cytokines TNF-α, IL- IL-8 content. 41 cases of healthy volunteers at the same period as the control group, comparative study. Results: The counts of CD3 + T cells, CD4 + T cells, CD4 + / CD8 + and CD16 + CD56 + NK cells in the observation group before treatment were significantly lower than those in the control group (P <0.05 (P0.05 or P0.001). The levels of inflammatory cytokines TNF-α, IL-6 and IL-8 in the observation group before treatment were significantly higher than those in the control group (P0.001) , After treatment was significantly lower (P <0.05 or P <0.001). CONCLUSION: Children with non-anaerobic pneumonia have poor cellular and humoral immunity and circulatory inflammatory response. Immunotherapy based on antibiotic therapy has a positive effect on the improvement of the disease.