Changes of 5' Terminal Nucleotides of PCR Primers Causing Variable T-A Cloning Efficiency

来源 :植物学报(英文版) | 被引量 : 0次 | 上传用户:felltwo23
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7<
其他文献
首次在栉孔扇贝(Chlamys farreri)中克隆了骨形态发生蛋白Ⅰ型受体(Bone morphogenetic protein type Ⅰ receptor,cfBMPR1)基因cDNA全长序列,该基因开放阅读框长1560 bp,编
介绍了在损伤的远端神经与近端神经间利用微电子神经桥实现神经信道桥接的技术,设计并实施了一个实验方案,验证了微电子模块实现神经信道桥接的可行性.以信源脊蟾蜍在化学刺
考虑填埋场衬垫系统材料间的接触特性,建立了复合衬垫系统直剪试验的有限元模型,采用ANSYS程序对填埋场复合衬垫系统的直剪试验过程进行了数值仿真分析。给出了不同法向力作用
介绍了Ni2O3/Fe2O3复合纳米微粒磁性液体的制备方法。研究了不同强度的外磁场作用下,平行于场方向照射的光束透过磁性液体样品后。其光透射率随磁场通、断而下降、上升的重复
Studies in model plants showed that SEPALLATA (SEP) genes are required for the identification of floral organs and the determination of floral meristems in Arab
中国媒介规制始于改革开放之初,目前存在条块分割,画地为牢,事业单位、企业经营双重角色,权力者对传媒规管随意性,媒介规制缺乏规范性和透明性等问题.为此,中国传媒规制的改
The random amplified mirosatellite polymorphism method was performed in a set of Tunisian fig landraces using eighteen primer combinations. Atotal of sixty thre
Global temperature has been increased by 0.6 ℃ over the past century and is predicted to increase by 1.4-5.8 ℃ by the end of this century. It is unclear what
Continuous isotope labeling and tracing is often needed to study the transformation, movement, and allocation of carbon in plant-soil systems. However, existing
A new protein, an approximately 59-kDa monomer containing iron atoms, was first isolated from the mutant strain DJ35 of Azotobacter vinelandli Lipmann. After an