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目的研究砷中毒后小鼠齿状回胶质原纤维酸性蛋白(GFAP)的变化。方法选取健康成年昆明小鼠80只,雌雄各半,分为对照组及慢性砷中毒高、中、低剂量组,每组20只,分别以蒸馏水、1/5 LD50、1/10 LD50、1/40LD50 As2O3连续灌胃3个月,根据其体质量变化随时调整用药剂量,采用Y-电迷宫检测各组小鼠学习记忆行为,采用免疫组织化学和蛋白印迹技术检测不同浓度砷中毒对小鼠齿状回部位GFAP表达的影响。结果 与正常对照组比较,高剂量砷中毒组学习、记忆Y-迷宫测试次数明显增多(P<0.05),小鼠齿状回GFAP阳性细胞明显增多(P<0.01),阳性反应产物平均光密度值增高(P<0.01),随砷中毒剂量的增加,小鼠齿状回GFAP蛋白含量随之增高(P<0.01)。结论 慢性砷中毒引起的学习记忆损伤,可能与齿状回神经胶质细胞反应性增生及GFAP表达增强有关。
Objective To study the changes of mouse dentate glial fibrillary acidic protein (GFAP) after arsenism. Methods Totally 80 healthy adult Kunming mice were randomly divided into control group and chronic arsenic poisoning high, medium and low dose groups, with 20 rats in each group. They were treated with distilled water, 1/5 LD50, 1/10 LD50, / 40LD50 As2O3 for 3 months, according to their body mass changes at any time to adjust the dose, the use of Y-electric maze test mice in each group learning and memory behavior, using immunohistochemistry and Western blotting detection of different concentrations of arsenic poisoning mice Effect of GFAP expression in dentate gyrus. Results Compared with the normal control group, the number of learning and memory Y-maze test in high-dose arsenic poisoning group was significantly increased (P <0.05), and the number of GFAP positive cells in dentate gyrus of mice significantly increased (P <0.01). The average optical density (P <0.01). With the increase of arsenic dose, the content of GFAP in mouse dentate gyrus increased (P <0.01). Conclusion The learning and memory impairment induced by chronic arsenic poisoning may be related to the hyperplasia of glial cells and the expression of GFAP.