论文部分内容阅读
目的 研究并比较槲皮素及其硫酸酯钠盐衍生物 (槲皮素单硫酸酯钠、槲皮素二硫酸酯钠 )对HL 6 0细胞生长的影响。方法 细胞存活力以台盼兰染色法测定 ;蛋白激酶CK 2活性以连接到CK 2底物上的 [γ 3 2 P]GTP的3 2 P放射活度来检测 ;细胞周期变化以流式细胞仪检测。结果 槲皮素、槲皮素单硫酸酯钠和槲皮素二硫酸酯钠均可抑制HL 6 0细胞的生长 ,槲皮素和槲皮素单硫酸酯钠对HL 6 0细胞生长的抑制作用差异无显著性 (P >0 0 5 ) ,槲皮素二硫酸酯钠的作用则比槲皮素弱得多 (P <0 0 1) ;槲皮素可抑制HL 6 0细胞中蛋白激酶CK 2的活性 ,而槲皮素单硫酸酯钠和槲皮1999 0 5 14收稿 ,1993 0 7 15修回 广东省自然科学基金资助课题 (No 940 5 86)和广东省高教厅重点学科资助课题 (No 93 0 4)作者简介 :翁 云 ,女 ,2 7岁 ,硕士 ;梁念慈 ,男 ,5 8岁 ,教授 ,博士生导师 ,广东医学院院长。研究方向 :生化药理学素二硫酸酯钠对HL 6 0细胞内CK 2活性无明显影响 ;槲皮素阻断HL 6 0细胞于G2 /M期 ,而槲皮素单硫酸酯钠和槲皮素二硫酸酯钠均阻断细胞于G0 /G1期。结论 槲皮素、槲皮素单硫酸酯钠和槲皮素二硫酸酯钠均为潜在的肿瘤细胞生长抑制剂 ,但它们的抑制机制却不相同 ,槲皮素 7 OH对维持槲皮素的功能有重要作用。
Objective To study and compare the effects of quercetin and its derivatives of sodium sulfate (sodium quercetin monosulfate, sodium quercetin disulfate) on the growth of HL 60 cells. Methods Cell viability was determined by trypan blue staining; protein kinase CK 2 activity was detected by the 3 2 P radioactivity of [γ 3 2 P]GTP linked to CK 2 substrate; cell cycle changes by flow cytometry Instrument detection. RESULTS: Quercetin, sodium quercetin monosulfate, and quercetin sodium bisulfate all inhibited the growth of HL 60 cells, and quercetin and quercetin monosodium sulfate inhibited the growth of HL 60 cells. There was no significant difference (P > 0 05). Quercetin disulfate was much weaker than quercetin (P <0 01). Quercetin inhibited protein kinase CK in HL 60 cells. 2 activity, while quercetin monosulfate sodium and suede were received in 1999 0 5 14 and 1993 0 7 15 was revisited back to Guangdong Provincial Natural Science Foundation of China (No 940 5 86) and Guangdong Provincial Department of High Education’s key subjects (No 93 0 4) About the author: Weng Yun, female, 2 7 years old, master degree; Liang Nianci, male, 5 years old, professor, doctoral tutor, dean of Guangdong Medical College. Research direction: Biochemical pharmacology Sodium disulfide has no significant effect on CK 2 activity in HL 60 cells; quercetin blocks HL 60 cells in G2 / M phase, while quercetin monosodium sulfate and suede Sodium dithionate blocked cells in the G0/G1 phase. Conclusions Quercetin, sodium quercetin monosulfate and sodium quercetin disulfate are all potential tumor cell growth inhibitors, but their inhibitory mechanisms are not the same. Quercetin 7 OH maintains quercetin Functions have an important role.