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作者选用5周龄的BALB/cAnNcR、C57BL/6、BALB/c.nu/nu(nude)和BALB/c.scid(SCID)4种小鼠为动物模型,用培养21小时的百日咳杆菌18323株气雾攻击小鼠,1小时后所有受试小鼠肺内均约有5×104CFU的活菌,用无菌技术切除感染小鼠的肺,测定不同感染时期的肺部活菌数。结果显示,攻击后2周BALB/c小鼠的肺内活菌数从攻击后1小时的4×104CFU/肺增至7×106CFU/肺,随后细菌数开始慢慢减少,至6～8周时完全清除;而作为对照的裸鼠(缺
The authors selected 4 weeks old BALB / cAnNcR, C57BL / 6, BALB / c.nu / nu (nude) and BALB / c.scid (SCID) as animal models and cultured 21 hours Bordetella pertussis 18323 Aerosol challenge mice, 1 hour after all the lungs of all mice were about 5 × 104CFU of live bacteria, aseptic technique was used to excise the lungs of infected mice, determination of viable count of lungs at different stages of infection. The results showed that the viable count in the lungs of BALB / c mice increased from 4 × 104 CFU / lung 1 hour after challenge to 7 × 10 6 CFU / lung 2 weeks after challenge, and then the number of bacteria started to decrease gradually to 6 to 8 weeks When completely cleared; and as a control nude mice (missing