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1型人类免疫缺陷病毒 ( HIV- 1 ) DNA的整合对于病毒在病人体内复制和持续存在至关重要。 Alu-聚合酶链反应 ( PCR)的基本原理是利用人类基因组中出现的许多 Alu重复序列 ,用一对能与 Alu序列结合的引物和一对 HIV特异性引物进行套式 PCR扩增 ,从而定量测定整合的 HIV基因。接?
The integration of type 1 human immunodeficiency virus (HIV-1) DNA is crucial for the virus to replicate and persist in the patient. The basic principle of Alu-Polymerase Chain Reaction (PCR) is the use of a number of Alu repeats appearing in the human genome, nested with a pair of primers capable of binding to the Alu sequence and a pair of HIV-specific primers for quantitative PCR amplification Determination of integrated HIV genes. Then?