用噁唑烷氮氧基硬脂酸(5—DSA)标记大鼠心肌细胞膜(SL),通过标记膜ESR光谱观察O_2~■)发生系统(X/XO)对SL膜流动性、Ca~(2+)泵和CK活性的影响。结果表明:X/XO引起SL膜流动性、Cu~(2+)泵和CK活性显著下降,均呈明显的量效关系;两个酶活性的下降与膜流动性降低密切相关;引起CK活性降低的X/XO浓度低于引起膜流动性降低的X/XO浓度。本研究表明:O_2~■)可引起心肌SL膜流动性降低,后者进一步导致膜Ca~(2+)泵活性下降。而O_2~■)引起CK活性下降所导致的能量代谢紊乱,可能会促进和加重膜流动性下降。这些变化可能是O_2~■)引起缺血再灌注性心肌损伤的早期环节。 Myocardial cell membrane (SL) was labeled with oxazolidine oxystearic acid (5-DSA), and the effect of X / XO on the membrane fluidity, 2+) pump and CK activity. The results showed that: X / XO induced SL membrane fluidity, Cu ~ (2 +) pump and CK activity were significantly decreased, both showed a significant dose-effect relationship; decreased activity of two enzymes and membrane mobility is closely related to reduce; The reduced X / XO concentration is lower than the X / XO concentration that causes a decrease in membrane fluidity. This study shows that: O2 ~ ■) can cause myocardial SL membrane fluidity decreased, which further lead to membrane Ca ~ (2 +) pump activity decreased. While O 2 ~ ■ caused by CK activity decreased energy metabolism disorders, may promote and aggravate the decline in membrane fluidity. These changes may be O_2 ~ ■) caused the early part of myocardial ischemia-reperfusion injury.