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以大豆品种粤春03-3为研究对象,采用水培的方式,在高低磷条件下,分别进行接种或不接种根瘤菌的处理。在大豆花期和成熟期分别测定大豆干重、氮磷浓度、根系苹果酸浓度和分泌速率,以研究结瘤对磷调控大豆生长及苹果酸合成和分泌的影响。结果表明:在低磷条件下,结瘤对大豆植株的干重、氮磷浓度均没有显著影响;在高磷条件下,结瘤能够显著提高植株地上部干重和氮浓度。在不结瘤条件下,磷对根系苹果酸浓度没有显著影响。但结瘤后,低磷明显增加了成熟期根系苹果酸的浓度。而且,结瘤显著提高了高磷条件下根系苹果酸的分泌速率。这些结果表明,结瘤影响了磷对大豆生长、根系苹果酸合成和分泌的调控。
With the soybean variety Yuechun 03-3 as the research object, the hydroponic method was used to treat rhizobia with or without inoculation under the condition of high and low phosphorus. The soybean dry weight, nitrogen and phosphorus concentration, root malic acid concentration and secretion rate were measured at the flowering and maturity stages of soybean to study the effects of nodulation on the growth of phosphorus-regulated soybean and the synthesis and secretion of malic acid. The results showed that the nodulation had no significant effect on the dry weight and nitrogen and phosphorus of soybean plants under low phosphorus condition. Under the high phosphorus condition, the nodulation could significantly increase the shoot dry weight and nitrogen concentration. Under non-nodulation conditions, phosphorus had no significant effect on the root malic acid concentration. However, after nodulation, low phosphorus significantly increased the concentration of malate at the mature stage. Moreover, nodulation significantly increased root exudation rate of malate under high phosphorus conditions. These results indicate that nodulation affects the regulation of phosphorus on soybean growth and root synthesis and secretion of malate.