论文部分内容阅读
通过对甲型N9亚型流感病毒神经氨酸酶(NA)核苷酸进化趋势的研究,进而探寻2013年新型H7N9亚型禽流感病毒产生的根源。本次研究选取美国国立生物技术信息中心(NCBI)的甲型N9亚型流感病毒的NA序列,采用生物软件ClustalX 2.0和MEGA 6.0建立进化树形图。对其欧亚分支,采用BEAST软件2.1.2和Datamonkey interface在线软件,计算和分析选择压力和进化速率。采用Bioedit软件对NA的氨基酸置换熵值计算并分析。系统进化树表明2013年新型H7N9亚型禽流感病毒可划分至现代欧亚分支之内,并且该分支的每位点每年核苷酸置换速率均值为3.8354×10-3,选择压力dN/dS均值为0.140413。16、19、40、53、81、84、112、256、335、359和401的氨基酸变异位点熵值>0.5。研究分析表明2013年新型H7N9亚型禽流感病毒的NA片段基因可能来自甲型N9亚型流感病毒逐步进化的结果,最早可追溯到1996年的A/duck/Siberia/700/1996(H11N9)流感毒株,新型H7N9亚型禽流感病毒来源不是来自外界环境压力引起突变的结果。
Based on the study of nucleotide evolution trend of neuraminidase (NA) of N9 influenza A virus, the origin of the new H7N9 subtype of avian influenza virus in 2013 was further explored. In this study, we selected the NA sequence of influenza A (N9) subtype influenza virus from the National Center for Biotechnology Information (NCBI) and used phylogenetic software ClustalX 2.0 and MEGA 6.0 to establish an evolutionary tree map. For its Eurasian branch, BEAST software 2.1.2 and Datamonkey interface online software were used to calculate and analyze selection pressure and evolution rate. The amino acid substitution entropy of NA was calculated and analyzed by Bioedit software. The phylogenetic tree showed that the new H7N9 subtype of avian influenza virus could be divided into modern Eurasian subfamilies in 2013, and the average nucleotide replacement rate per site of this sub-site was 3.8354 × 10-3 in 2013, and the selection pressure dN / dS average Amino acid variation site entropic value> 0.5 for 0.140413.16, 19,40,53,81,84,112,256,335,359 and 401. Research and analysis showed that the NA fragment gene of the new H7N9 subtype of avian influenza virus in 2013 may come from the gradual evolution of the influenza A (N9) subtype influenza virus, which can be traced back to 1996 in the A / duck / Siberia / 700/1996 (H11N9) The strain, the source of the new H7N9 subtype of avian influenza virus, is not the result of a stress-induced mutation in the outside world.