论文部分内容阅读
目的 研究川芎嗪 (TMP)对人多形核白细胞 (PMN)呼吸爆发期间氧消耗和活性氧自由基的影响。方法 用PMA刺激PMN呼吸爆发产生的活性氧自由基 ,黄嘌呤 /黄嘌呤氧化酶体系产生的超氧阴离子 (O2 -)和Fenton反应产生的羟自由基 (OH-)为模型。用电子自旋共振自旋捕集技术 ,自旋探针氧测定法和化学发光法研究TMP对活性氧自由基O2 -和OH-的清除作用及对PMN呼吸爆发时氧消耗的影响。结果 TMP(0 .18mol·L-1)对两种水溶液体系产生的O2 -和OH-的清除率分别达 10 0 %和 44 % ,也能显著清除细胞体系产生的活性氧自由基 ,抑制它的化学发光过程 ,但对PMN呼吸爆发时的氧消耗无明显影响。结论 TMP不影响PMN呼吸爆发时的氧化代谢 ,但能通过清除氧自由基防止激活PMN对组织的损伤。
Objective To study the effects of tetramethylpyrazine (TMP) on oxygen consumption and reactive oxygen species during respiratory burst in human polymorphonuclear leukocytes (PMN). Methods PMA was used to stimulate reactive oxygen species produced by respiratory bursts in PMNs, superoxide anion (O2 -) produced by xanthine/xanthine oxidase system, and hydroxyl radical (OH-) produced by Fenton reaction. The effects of TMP on the scavenging of active oxygen radicals O2 - and OH- and on the oxygen consumption of PMN respiratory bursts were investigated by electron spin resonance spin trapping technique, spin probe oxygen measurement and chemiluminescence. Results The O2 - and OH- clearance rates of TMP (0.18 mol·L-1) for the two aqueous systems reached 100% and 44%, respectively, which also significantly cleared the reactive oxygen species produced by the cell system and inhibited it. The chemiluminescence process did not have a significant effect on the oxygen consumption during PMN respiratory bursts. Conclusions TMP does not affect the oxidative metabolism of PMN during respiratory bursts, but it can prevent PMN from damaging tissue by removing oxygen free radicals.