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目的 :本实验观察黄芪甲甙对炎症介质组胺引起的血管内皮通透性增加的作用。方法 :从新生牛主动脉分离获得单个内皮细胞 ,培养于微孔滤膜上直至形成致密单层。通过灌流 hanks液或含 5g.L-1白蛋白的 hanks液后 ,测定经 1 0 -4 M组胺溶液处理或经 1 0 -4 M组胺溶液加 1 0 -4 g.ml-1黄芪甲甙处理的液体滤过系数 (Kf)、液体滤过流量 (Jv)和蛋白质渗透压反射系数 (σ)。结果 :内皮单层经组胺处理后 ,Kf和 Jv降低 ,σ升高 ,而黄芪甲甙能够抑制因组胺造成的 Kf和 Jv降低 ,以及σ升高。结论 :表明组胺能够引起血管内皮单层通透性的增加 ,而黄芪甲甙能够减轻组胺造成的内皮单层通透性的增加 ;其作用机制需要进一步研究。
Objective : This experiment was to observe the effect of Astragaloside IV on histamine-induced vascular endothelial permeability increase. METHODS: Single endothelial cells were isolated from bovine aorta and cultured on microporous membranes until a dense monolayer was formed. After perfusion of the Hanks solution or Hanks’ solution containing 5 g.L-1 albumin, determine after treatment with 1 0 -4 M histamine solution or with 1 0 -4 M histamine solution plus 1 0 -4 g.ml -1 jaundice Membrane treated fluid filtration coefficient (Kf), liquid filtration flow rate (Jv) and protein osmotic pressure reflection coefficient (σ). RESULTS: After the histamine treatment of the endothelial monolayer, Kf and Jv decreased and σ increased, whereas Astragaloside inhibited the decrease of Kf and Jv caused by histamine, and the increase of σ. Conclusions: Histamine can increase the permeability of vascular endothelial monolayer, and Astragaloside can reduce the increase of histamine-induced endothelial monolayer permeability. Its mechanism of action needs further study.