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目的建立甘草次酸-丹参酮ⅡA复方脂质体(GT-Lip)包封率的测定方法。方法分别采用葡聚糖凝胶过滤法、低速离心法分离脂质体和游离药物。以HPLC法为分析手段,比较该2种方法包封率的测定结果。结果葡聚糖凝胶G-50(M)对脂质体有一定吸附,不适合GT-Lip包封率的测定;低速离心法能使GT-Lip脂质体与游离药物有效分离,经HPLC法测定,丹参酮ⅡA(TSN)与甘草次酸(GA)平均包封率分别达到(81.50±0.76)%和(98.63±0.90)%。结论 HPLC法与低速离心法相结合可快速、有效用于甘草次酸-丹参酮ⅡA复方脂质体的包封率测定。
Objective To establish a method for determination of encapsulation efficiency of glycyrrhetinic acid-tanshinone Ⅱ A compound liposomes (GT-Lip). Methods Dextran gel filtration and liposome and free drug were separated by low speed centrifugation. HPLC method was used as an analytical method to compare the results of the two methods encapsulation efficiency. Results Sephadex G-50 (M) had some adsorption on liposomes and was not suitable for the determination of GT-Lip encapsulation efficiency. Low-speed centrifugation could effectively separate GT-Lip liposome from free drug. The average entrapment efficiencies of TSN and GA were (81.50 ± 0.76)% and (98.63 ± 0.90)%, respectively. Conclusion The combination of HPLC and low-speed centrifugation can be used to determine the entrapment efficiency of glycyrrhetinic acid-tanshinone Ⅱ A liposomes rapidly and effectively.