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目的:探讨Notch 3受体特异性沉默对急性T淋巴细胞白血病的影响。方法:运用survivin启动子介导的RNA干涉方法特异性地封闭急性T淋巴细胞白血病细胞SupT1中Notch 3基因的表达。结果:survivin启动子介导的RNA干涉系统能特异而高效封闭肿瘤细胞内源Notch 3基因的表达,Notch 3基因表达的下调能够使肿瘤细胞的增殖活性降低,凋亡明显增加。结论:Notch 3基因沉默可有效抑制白血病细胞的增殖,并促进其凋亡,为急性T淋巴细胞白血病相关基因功能研究及靶向性治疗探索了一种新策略。
Objective: To investigate the effect of Notch 3 receptor-specific silencing on acute lymphoblastic leukemia. Methods: The expression of Notch 3 gene in SupT1 cells of acute T lymphocytic leukemia cells was specifically blocked by survivin promoter-mediated RNA interference. Results: The RNA interference system mediated by survivin promoter could specifically and efficiently block the expression of endogenous Notch 3 gene in tumor cells. The down-regulation of Notch 3 gene expression could decrease the proliferation activity and increase the apoptosis rate of tumor cells. Conclusion: Notch 3 gene silencing can effectively inhibit the proliferation and promote the apoptosis of leukemia cells, and explore a new strategy for the study of the function and target therapy of acute T lymphocyte leukemia related genes.