论文部分内容阅读
为明确氟唑菌酰胺和吡唑醚菌酯在芒果上的残留行为,于2012和2013年在中国广东省和广西自治区进行了氟唑菌酰胺和吡唑醚菌酯在芒果上的田间残留及消解动态试验,建立了芒果中氟唑菌酰胺及吡唑醚菌酯残留量的高效液相色谱检测方法。样品用丙酮提取,乙酸乙酯液-液分配萃取,弗罗里硅土柱层析净化,高效液相色谱-二级管阵列紫外检测器检测,外标法定量。结果表明:氟唑菌酰胺和吡唑醚菌酯在芒果上的消解半衰期分别为7.2~9.1和8.0~11.0 d;采用42.4%吡唑醚菌酯·氟唑菌酯胺悬浮剂(SC),分别按有效成分200和300 mg/L的剂量于幼果期开始施药,施药3~4次,施药间隔期为10~15 d,距最后一次施药后7和14 d采样测定,芒果中氟唑菌酰胺和吡唑醚菌酯的残留量分别为0.004~0.053和0.004~0.072 mg/kg。其中,吡唑醚菌酯残留量符合中国制定的最大残留限量(MRL)标准(0.05 mg/kg),根据试验结果,建议中国可将氟唑菌酰胺在芒果上的MRL值暂定为0.2 mg/kg。
In order to clarify the residual behavior of PFZ and pyraclostrobin in mango, field residues of PFZ and pyraclostrobin in mango were conducted in Guangdong and Guangxi autonomous regions in 2012 and 2013, respectively. Digestion dynamic test, the establishment of mangroves in pyronaridine and pyraclostrobin residues residues by high performance liquid chromatography detection method. The samples were extracted with acetone, extracted with ethyl acetate liquid - liquid, and purified by Florisil column chromatography. The samples were determined by HPLC - diode array UV detector and quantified by external standard method. The results showed that the digestion half-lives of difloxacin and pyraclostrobin in mangoes were 7.2-9.1 and 8.0-11.0 days, respectively. With 42.4% pyraclostrobin and triflusal suspension (SC) The effective doses of 200 and 300 mg / L were applied in the young fruit stage respectively. The application rate was 3 to 4 times, the interval of application was 10 to 15 days, and the sampling time was 7 and 14 days after the last application. The residues of PFZ and pyraclostrobin in mango were 0.004 ~ 0.053 and 0.004 ~ 0.072 mg / kg, respectively. Among them, the residues of pyraclostrobin meet the MRL standard (0.05 mg / kg) established in China. According to the test results, it is suggested that MRL value of halozolomide in mango may be tentatively set as 0.2 mg / kg.