论文部分内容阅读
目的 研究TIMP 2基因转染胃癌细胞后的生物学行为变化。方法 采用基因转染的方法将人全长TIMP 2cDNA基因转染入人胃癌SGC 790 1细胞系中 ,利用层粘连蛋白 (LN)对肿瘤细胞的诱导迁移作用 ,在体外侵袭模型中观察转染基因细胞侵袭能力的改变。结果 转染TIMP 2基因的SGC 790 1细胞侵袭能力 [33 1± 11 3细胞 h]明显低于未转染组 [( 135 7± 2 1 6 )细胞 h ,P <0 0 1],肿瘤细胞在LN上的粘附能力受到抑制 ,转染组 ( 417 7± 2 1 5 )低于未转染组 ( 82 9 4± 45 7) (P <0 0 5 )。且细胞的增殖能力受到限制。结论 TIMP 2基因可阻抑层粘连蛋白对瘤细胞的诱导侵袭影响 ,参与瘤细胞转移的阻断机制
Objective To study the biological behavior of TIMP 2 gene transfected gastric cancer cells. METHODS: Human full-length TIMP 2 cDNA gene was transfected into human gastric cancer SGC 790 1 cell line by gene transfection method. The effect of laminin (LN) on tumor cell migration was observed. The transfected gene was observed in an in vitro invasion model. Changes in cell invasive ability. Results The invasive ability of SGC 790 1 cells transfected with TIMP 2 gene was significantly lower than that of non-transfected cells [(135 7±2 1 6) cells h, P <0 01]. Adhesion capacity on LN was inhibited, and the transfection group (417 7±2 1 5) was lower than the non-transfected group (82 9 4± 45 7) (P < 0 05). And the cell’s proliferation ability is limited. Conclusion TIMP 2 gene can inhibit the invasive effect of laminin on tumor cells and participate in the blocking mechanism of tumor cell metastasis