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目的 探讨 85 %高浓度氧暴露对早产新生大鼠肺组织角化细胞生长因子 (KGF)mRNA及增殖细胞核抗原 (PCNA)动态表达的影响。方法 早产SD大鼠生后 1d随机分为空气组、高氧组。高氧组持续暴露 85 %氧气中 ,空气组置于同一室内常压空气中。两组分别于 1、4、7、10、14和 2 1d时 ,提取肺组织RNA ,采用半定量逆转录聚合酶链反应 (RT PCR)测定KGFmRNA ;同时应用免疫组化检测肺组织切片中PCNA。结果 (1)与空气组相比 ,高氧 1d时 ,KGFmRNA表达无明显差异 (P >0 0 5 ) ;4d时明显增强 ,较空气组增加 3 8倍 (P <0 0 0 1) ;其后开始下降 ,7d时较空气组增加 1 8倍 (P<0 0 5 ) ;10d时两组间无明显差异 (P >0 0 5 ) ;14d时较空气组降低 1 5倍 (P <0 0 1) ,2 1d时复又增高 ,较空气组增加 1 15倍 (P <0 0 1)。 (2 )空气组早产新生大鼠生后 14d内肺组织中均有PCNA表达 ,并且PCNA阳性细胞 90 %以上定位于气道和肺泡上皮细胞 ,2 1d时几乎检测不出其表达 ;与空气组比较 ,高氧组 1～ 4d时肺组织中PCNA表达明显减弱 ,7～ 10d时增强且部分间质细胞也有表达 ,2 1d时PCNA阳性细胞以间质细胞为主。结论 85 %高氧暴露可促进早产新生大鼠肺组织KGFmRNA及PCNA表达
Objective To investigate the effects of 85% oxygen exposure on dynamic expression of KGF mRNA and proliferating cell nuclear antigen (PCNA) in lung of premature newborn rats. Methods The preterm SD rats were randomly divided into air group and hyperoxia group 1 day after birth. Oxygen continued exposure of 85% of the oxygen group, the air group placed in the same indoor atmosphere of air. At 1, 4, 7, 10, 14 and 21 days, the lung tissue RNA was extracted from the two groups, and KGF mRNA was detected by semi-quantitative reverse transcription polymerase chain reaction (RT PCR). Immunohistochemistry was used to detect PCNA . Results (1) Compared with air group, the expression of KGF mRNA had no significant difference (P> 0.05) at 1 day of hyperoxia and significantly increased at 4 days, which was 38 times more than that of air group (P <0.01 01) (P <0 05). There was no significant difference between the two groups on the 10th day (P> 0.05), and on the 14th day, it was 15 times lower than the air group (P <0 05) 0 1), increased again on 21d, and increased 1 15 times (P <0.01) over air group. (2) The expression of PCNA in the lung tissue of the premature newborn rats of the air group within 14 days after birth, and more than 90% of the PCNA positive cells located in the airway and alveolar epithelial cells, and almost no detectable expression was found 21 days later. Compared with the air group Compared with the control group, the expression of PCNA in the lung tissue of the rats in the hyperoxia group was significantly weakened at 1 ~ 4d, and enhanced at 7-10d. Some of the interstitial cells were also expressed in the hyperoxia group. The majority of PCNA positive cells were mainly stromal cells on the 21d. Conclusion 85% hyperoxia exposure can promote the expression of KGFmRNA and PCNA in the lungs of premature neonatal rats