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目的和方法研究7-hydroxystaurosporine(UCN-01)对转移性前列腺肿瘤DU-145细胞浸润和转移的影响.用体外转移和创伤法检查细胞浸润和转移,用Westernblotting法检查蛋白质的表达.结果UCN-01在非毒性剂量下(100nmol·L-1)明显地抑制DU-145细胞浸润和转移.而且,UCN-01这种抗肿瘤浸润和转移功能与它增加细胞-细胞粘连分子E-cadherion的表达有关.结论这些实验首次证实UCN-01能抑制人前列腺肿瘤细胞的浸润和转移.UCN-01的临床应用可能更有效地控制前列腺肿瘤转移.“,”Aim and Methods To investigate the effect of UCN-01(7-hydroxystaurosporine) on cell migration and invasion ability of DU-145, an invasive human prostate cancer cell line.Results It was found that UCN-01 at non-cytotoxic doses (100 nmol· L-1) significantly inhibited prostate cancer DU-145 cell invasion and migration behaviors.Moreover, this anti-invasion and migration activity of UCN-01 was associated with an up-regulation of cell adhesion molecule E-cadherin. Conclusion These results indicate for first time that UCN-01 inhibits the invasion and migration of human prostate cancer cells.Thus, clinical application of UCN-01 may contribute to the potential benefit for suppression of prostate cancer invasion and metastasis.