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目的探讨5-氨基乙酰丙酸(5-ALA)介导的光动力学对人脑胶质瘤U251细胞的治疗作用方法使用荧光显微镜及共聚焦显微镜检测5-ALA所产生的光敏剂原卟啉(PpIX)在U251细胞中的定位。将5-ALA加入U251细胞中,随之以635nm的激光辐射,使用MTT法测定细胞生存率结果5-ALA与U251细胞共培养可以产生光敏剂原卟啉。原卟啉弥散地分布在U251细胞的胞浆中,胞核区未见分布。5-ALA介导的光动力对人脑胶质瘤U251细胞的杀伤作用随着5-ALA与U251细胞孵育时间的延长及5-ALA浓度的增加而增加,但在孵育6h、5-ALA浓度为2mmol/L时达饱和。而单用5-ALA或单纯激光辐射,对U251细胞无明显的杀伤作用。结论5-ALA介导的光动力治疗是很有前途的人脑胶质瘤治疗方法,5-ALA与U251细胞孵育的最佳时间为6h,最佳5-ALA药物浓度为2mmol/L
Objective To investigate the therapeutic effect of 5-aminolevulinic acid (5-ALA) -mediated photodynamic therapy on human glioma U251 cells.Methods Fluorescence microscopy and confocal microscopy were used to detect the presence of photosensitizer protoporphyrin (PpIX) in U251 cells. 5-ALA was added to U251 cells followed by laser irradiation at 635 nm. Cell viability was determined using the MTT method. Results 5-ALA and U251 cells were co-cultured to produce the photosensitizer protoporphyrin. Protoporphyrin diffusely distributed in the cytoplasm of U251 cells, no distribution in the nucleus. The killing effect of 5-ALA-mediated photodynamic therapy on human glioma U251 cells increased with the incubation time of 5-ALA and U251 cells and the increase of 5-ALA concentration. However, the concentration of 5-ALA 2mmol / L up to saturation. However, 5-ALA alone or laser irradiation alone had no obvious killing effect on U251 cells. Conclusions 5-ALA-mediated photodynamic therapy is a promising method for the treatment of human glioma. The optimal time for incubation with 5-ALA and U251 cells is 6h, and the optimal 5-ALA concentration is 2mmol / L